Stanislav Pepeliaev Ph.D.
Biotech Consultancy
I am a versatile and experienced team leader with deep-rooted expertise in chemistry, biology, and team management, honed through a dedicated focus on biotechnology. Over the course of my career, I have cultivated a strong reputation for driving innovation and fostering collaboration within multidisciplinary teams. My primary scientific interests lie in industrial biotechnology, high-throughput methodologies, and automation, where I thrive in developing cutting-edge solutions to complex challenges.
Building and leading high-efficiency research teams is at the core of my professional ethos, ensuring the seamless integration of diverse skill sets to achieve impactful results. I am passionate about translating scientific breakthroughs into practical, scalable applications, contributing to advancements that shape the future of biotechnology. With a proactive approach and a commitment to excellence, I deliver expertise and leadership that empower organizations to succeed in a competitive and ever-evolving industry.
Services
Tech Transfer
With a strong background in biotechnology and biopharmaceuticals, I specialize in facilitating seamless technology transfer processes. From early-stage development to industrial-scale manufacturing, I help organizations navigate the complexities of biotechnological innovation. My expertise ensures that processes are not only scalable but also compliant with industry regulations, maintaining product integrity and efficiency at every stage. Whether you're transitioning laboratory discoveries into commercial applications or improving existing workflows, I offer tailored solutions to meet your unique needs. Together, we can turn your cutting-edge research into tangible, market-ready successes.
Technical Due Diligence
I offer expert technical due diligence services in biotechnology, ensuring thorough evaluation and risk assessment of technologies, processes, and operations. With a deep understanding of bioprocess development and industry compliance, I provide actionable insights to support investment decisions, partnerships, and strategic planning. My expertise ensures that every aspect, from scalability to regulatory alignment, is meticulously reviewed to minimize risks and maximize potential. Whether you’re an investor, a startup, or an established organization, I deliver precise and informed assessments to help you make confident, data-driven decisions.
Research to Manufacture interface
I specialize in guiding the seamless transition of biotechnological innovations from research and development to full-scale manufacturing. By addressing key challenges in scaling up, such as process robustness, cost-efficiency, and regulatory readiness, I ensure a smooth pathway to commercialization. My expertise supports clients in transforming laboratory breakthroughs into viable, market-ready products with minimized risks and optimized workflows.
Biologics Process Development
With extensive experience in bioprocess development, I design and optimize both upstream and downstream processes tailored to meet the unique demands of each product. From selecting the right expression systems to refining purification strategies, I focus on developing scalable, compliant, and efficient processes. My approach ensures high-quality outcomes that align with industry standards and client-specific goals.
My Journey
Independent consultant in biotechnology
Since July 2024
Currently, as a Biotechnology Consultant, I collaborate with leading organizations to address complex challenges in bioprocess development, technology transfer, scale-up, and analytical assay development. My work spans diverse areas, including virus-like particles, recombinant proteins, polysaccharides, and DNA production. From optimizing a polysaccharide isolation process to leading the development and tech transfer of automated bioprocesses, my contributions have consistently delivered cost savings, efficiency improvements, and seamless transitions to GMP manufacturing.
Small research facility director
University of Oxford | 2021 - 2024
I led the BioProcess and Analytical Development Small Research Facility (BiPAD SRF) at the University of Oxford. There, I managed a multidisciplinary team supporting academic research teams in translating novel therapeutics into clinical trials. My tenure saw the successful development and tech transfer of GMP-compliant bioprocesses for preclinical and Phase I/II studies, optimization of adenoviral and CHO-K1 cell production platforms, and the design of a state-of-the-art process development laboratory. Among my proudest achievements was the development of the first gonococcal challenge agent manufacture process in Europe for human infection model studies.
Principal Scientist
Ipsen | 2018 - 2021
I bridged the gap between research and development for toxin-based therapeutic products. My innovative approach to high-throughput upstream process development and automation earned patents. Among the other achievements, one process oprimisation led to the improvement of target protein solubility sevenfold and another increased throughput from six to 24 candidate molecules per week being tested.
Head of the Biotechnological Laboratory
Contipro | 2016 - 2018
I established a multidisciplinary laboratory from the ground up, overseeing everything from strategy development to tendering and equipment setup. My efforts in high-throughput strain engineering and process analytical technology implementation directly boosted company revenues and reduced batch failure rates.
Portfolio
MaxCyte
SIMCELL
Thermo Fisher Scientific
Chiesi
Glen Clova Scientific
EnzymIt
Patents
1. Controlling operation of a bioreactor vessel. WO2022013560 · Issued Jul 15, 2020
2. Composition comprising an iodide and a derivative of hyaluronic acid with an oxidative effect, method of preparation thereof and use thereof. CZ WO2020038501A1 · Issued Feb 27, 2020
3. Method of determination of hyaluronic acid. EU WO2018166545 (A1) · Issued Sep 20, 2018
4. Thermally stable hyaluronidase enzyme, process of its preparation, use thereof and process for preparing low-molecular hyaluronan. CZ CZ2014564A3 · Issued Aug 21, 2014
Publications
1. Pepeliaev S., Ehebauer M.T. (2022) Cultivation of microbial cultures at high cell density in microbioreactor fermentations to increase protein yield. Biotechnol Appl Biochem. 2022; 1– 7.
2. Pepeliaev, S. (2021). Automated fermentation platform for toxin-based therapeutics. Toxicon, 190, S1, S59.
3. Jilková, J., Liskova, P., Pepeliaev, S., Černý, Z., Chatzigeorgiou, S., Klíč, V., Čihák, M., & Velebný, V. (2018). Hyaluronic acid oligosaccharides are substrate for β-N-acetylhexosaminidase from Streptomyces plicatus. New Biotechnology, 44.
4. Pepeliaev, S., Černý, Z., Jilková, J., Čihák, M., Chatzigeorgiou, S., Klíč, V., & Velebný, V. (2018). The mechanism of natural competence loss in animal pathogen Streptococcus zooepidemicus. New Biotechnology, 44.
5. Kašparová, J., Korecká, L., Pepeliaev, S., Bílková, Z., Smirnou, D., Velebný, V., & Česlová, L. (2018). Magnetic macroporous bead cellulose functionalised with recombinant hyaluronan lyase for controllable hyaluronan fragmentation. Process Biochemistry, 72, 105-111.
6. Pepeliaev, S., Hrudíková, R., Jilková, J., Pavlík, J., Smirnou, D., Černý, Z., & Franke, L. (2017). Colorimetric enzyme-coupled assay for hyaluronic acid determination in complex samples. European Polymer Journal, 94, 460-470.
7. Čožíková, D., Šílová, T., Moravcová, V., Šmejkalová, D., Pepeliaev, S., Velebný, V., & Hermannová, M. (2017). Preparation and extensive characterization of hyaluronan with narrow molecular weight distribution. Carbohydrate polymers, 160, 134-142.
8. Franke, L., Smirnou, D., Pepeliaev, S., Černý, Z., Hrudíková, R., & Velebný, V. (2016). N-Acetylglucosamine as a signalling molecule triggering hyaluronic acid increase in Streptococcus zooepidemicus. New Biotechnology, 33.
9. Smirnou, D., Bobková, L., Krcmár, M., Hermannová, M., Franke, L., Pepeliaev, S., Jilková, J., & Velebný, V. (2016). Hyaluronan degrading enzymes in fungi. New Biotechnology, 33.
10. Smirnou, D., Krcmár, M., Kulhánek, J., Hermannová, M., Bobková, L., Franke, L., Pepeliaev, S., & Velebný, V. (2015). Characterization of Hyaluronan-Degrading Enzymes from Yeasts. Applied Biochemistry and Biotechnology, 177, 700-712.
11. Tlustá, M., Krahulec, J., Pepeliaev, S., Franke, L., Černý, Z., & Jilková, J. (2012). Production of Hyaluronic Acid by Mutant Strains of Group C Streptococcus. Molecular Biotechnology, 54, 747-755.
12. Pepeliaev, S., Krahulec, J., Tlustá, M., Černý, Z., Jílková, J. (2012). Expression and purification of the light chain of human enteropeptidase in E. coli. Minerva Biotecnologica 2012 June;24(2):42-52.
13. Pepeliaev, S., Krahulec, J., Černý, Z., Jilková, J., Tlustá, M., & Dostálová, J. (2011). High level expression of human enteropeptidase light chain in Pichia pastoris. Journal of biotechnology, 156 1, 67-75.
14. Krahulec, J., Hyršová, M., Pepeliaev, S., Jilková, J., Černý, Z., & Machálková, J. (2010). High level expression and purification of antimicrobial human cathelicidin LL-37 in Escherichia coli. Applied Microbiology and Biotechnology, 88, 167-175.
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